Histologically compatible tissue imaging

A recent development in DESI-MS imaging has been the introduction of “morphologically friendly” dimethylformamide (DMF)-based solvent systems, thatallows  imaging to be performed while maintaining the morphology of the tissue section intact. Therefore, DESI-MS imaging and histochemical analysis can be performed on the same tissue section, allowing accurate correlation between molecular signatures and disease state. The morphologically friendly solvents also allow MALDI imaging to be performed on the same tissue section for protein analysis after DESI. This means that chemical information on lipids and proteins can be achieved in the same tissue section, which can then be matched to morphological information by histochemistry.